The validity of the method yielded the following results: relative sensitivity of 95%, relative specificity of 90%, relative accuracy of 93%, positive predictive value of 95%, and negative predictive value of 90%. The limit of detection for detecting organophosphates and carbamates was in the range of 0.002–0.877 ppm. The specificity and purification factor of the produced ChE were 1.54 µM/min/mg and 1.43-fold, respectively. In this study, the Km and Vmax values for cricket cholinesterase were 3.75 mM and 35 µM/min, respectively. The same enzyme activity was observed at 25 ☌ and 30 ☌, which was greater than at 35 ☌ and 40 ☌. Using a buffer with a pH of 8.0 resulted in the greatest activity after 5 min. The results indicated that acetylthiocholine iodide was more active than butyrylthiocholine iodide. The method’s parameters, including substrate specificity, kinetics, and incubation time, were optimized. The purpose of this study was to develop an in-house colorimetric method for detecting organophosphate and carbamate residues using cricket cholinesterase that can be used as a low-cost, simple-to-use, and widely used test. The assessment of residues in food materials is required for consumer and agricultural worker health protection. Pesticides such as organophosphates and carbamates are used to control pests, leaving residues on fruits and vegetables.
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